In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant
Abstract
Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of 1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents.
Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
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Chawla, A.S., Krishnan, T.R., Jackson, A.H. and Scalabrin, D.A., 1988, Alkaloidal Constituents of Erythrina variegate bark, Planta Med., 546, 526-528. CrossRef
Herlina, T., Nasrudin, Supratman, U., Subarnas, A., Sutardjo, S. and Hayashi, H., 2008, An Isoflavonoid, Warangalone from the Stem Bark of Dadap Ayam (Erythrina variegata), Journal of Basic Sciences, 9(1), 45-47. Link
Herlina, T., 2009, Anticancer Compounds from Dadap Ayam (Erythrina variegata), Indones. J. Cancer, 3(4), 151-154. Link
Heyne, K., 1987, The Useful Indonesian Plants, Research and development agency, Ministry of forestry, Jakarta, Indonesia, pp. 1029-1031.
Skehan, P.R., Storeng, D., Scudiero, A., McMahon, D., Vistica, J.T., Warren, H., Boskesch, S., Kenney, and Boyd, M.R., 1990, Journal Natural Product., 82, 13.
Supratman, U., Fujita, T. and Hayashi. H., 2000, Paralytic alkaloid from Erythrina subumbrans (Leguminosae), J. Biol. Sci, 6, 7-16.
Tanaka, H., Toshihiro, T. and Etoh, H., 1998, Two Pterocarpans from Erythrina orientalis, Phytochemistry, 47(3), 475-477. CrossRef
Tanaka, H., Etoh, H., Shimizu, H., Makita, T. and Tateishi, Y., 2000, Two New Isoflavonoids from Erythrina variegate, Planta Med., 66(6), 578-579. CrossRef
Tanaka, H., Hirata, M., Etoh, H., Watanabe, N., Shimizu, H., Ahmad, M., et al., 2002, Two diphenylpropan-1,2-diol Syringates from the Roots of Erythrina variegate, J. Nat. Prod., 65(12), 1933-1935. CrossRef
Tanaka, H., Hirata, M., Etoh, H., Shimizu, H., Sako, M., Murata, J., et al., 2003, Eryvarins F and G, two 3-phenoxychromones from the roots of Erythrina variegata , Phytochemistry, 62(8), 1243-1246. Link
Tyagi, A.K., Singh, R.P., Agarwal, C., Chan, D.C. and Agarwal, K., 2002, Silibinin Strongly Synergizes Human Prostate Carcinoma DU145 Cells to Dox-induced Growth Inhibition, G2-M Arrest and Apoptosis, Clin. Cancer Res., 8(11), 3512-3519. Link
Tyagi, A.K., Agarwal, C., Chan, D.C. and Agarwal, K., 2004, Synergistic Anti-Cancer Effect of Silibinin with Conventional Cytotoxic Agents Doxorubicin, Cisplatin and Carboplatin Against Human Breast Carcinoma MCF-7 and MDA-MB468 Cells, Oncol. Rep., 11(2), 493-499. Link
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss3pp286-290
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