Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells

Nur Ismiyati, Dyaningtyas Dewi Pamungkas Putri, Siska Andrina Kusumastuti, Rifki Febriansyah

Abstract


Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effect on T47D cells with IC50 value of 65 µg/ml. Moreover, EEU 50µg/ml and 100µg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.

Keywords: Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis


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References


Handayani S., 2006, Efek Sitotoksik Ekstrak Kloroform, Etil Asetat, dan Etanol Daun Dewandaru (Eugenia uniflora Linn.) terhadap Sel Hela, Essay, Fakultas Farmasi Universitas Muhamadiyah Surakarta, Surakarta.

Harborne, J.B., 1987, Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan, Bandung: ITB.

Hutapea, J.R., 1994, Inventaris Tanaman Obat Indonesia, 3rd Edition, Departemen Kesehatan RI dan Badan Penelitian dan Pengembangan Kesehatan, 29-30.

Kampa, M., Alexaki, VI, Notas, G., Nifli, A.P., Nistikaki, A., Hatzoglou, A., et al., 2003, Antiproliferative and Antiapoptotic Effects of Selective Phenolic Acids on T47D Human Breast Cancer Cells: Potential Mechanism of Action, Breast Cancer Res., 6(2), 63-74. CrossRef

King, R.J.B., 2000, Cancer Biology, 2nd Edition., London: Pearson Eduation Limited.

Lee, M.H., Chiou, J.F., Yen, K.Y. and Yang, L.L., 2000, EBV DNA Polymerase Inhibition of tannins from Eugenia uniflora, Cancer Lett., 154(2), 131–136. Link

McGahon, A. J., Martin, S.J., Bissonnette, R.P., Mahboubi, M., Shi, Y., Mogil, R.J., Nishioka, W. K., and Green, D.R., 1995, The End of the (Cell) Line: Methods for the Study of Apoptosis in Vitro. In: Schwartz, L.M. and Osborn, B.A., ed., Cell Death, Academic Press, San Diego.

Nam, S., Smith, D.M. and Dou, Q.P., 2001, Tannic Acid Potently Inhibits Tumor Cell Proteasome Activity, Increases p27 and Bax Expression, and Induces G1 Arrest and Apoptosis, Cancer Epidemiol. Biomarkers Prev., 10(10), 1083-1088. Link

Rahardja, K. and Tjay, T.H., 2002, Obat-Obat Penting Khasiat, Penggunaan, dan Efek-Efek Sampingnya, 5th Edition, Jakarta: P.T. Elex Media Komputindo Kelompok Gramedia.

Schmeda-Hirschmann, G., Theoduloz, C., Franco, L., Ferro, E. and de Arias, A.R., 1987, Preliminary Pharmacological Studies on Eugenia Uniflora Leaves: Xanthine Oxidase Inhibitory Activity, J. Ethnopharmacol., 21(2), 183-186. Link

Taraphdar, A.K., Roy, M. and Bhattacharya, R.K., 2001, Natural Products as Inducers of Apoptosis: Implication for Cancer Theraphy and Prevention, Curr. Sci., 80(11), 1387–1396. Link

Tjindarbumi, D. and Mangunkusumo, R., 2001, Cancer in Indonesia, Present and Future, Jpn. J. Clin. Oncol., 32(Supplement 1), S17-S21. Link

Wagner, H. and Bladt, S., 1996, Plant Drug Analysis The Thin Layer Chromatography Atlas, 2nd Edition, Berlin: Springer Verlag.




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev3iss2pp370-375

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