Anti-Inflammatory Effect of Ethanolic Extract of Curcuma aeruginosa Roxb Rhizome, Morinda Citrifolia Fruit and Apium graveolens Leaf on Lipopplysaccharide-induce RAW 264.7 Cell Lines
Abstract
Curcuma aeruginosa Roxb., Morinda citrifolia and Apium graveolens are Indonesian plants which have been used in traditional medicine as an antihelmintic, antimicrobial, antiinflammation and antioxidant. In this study, the antiinflammatory activity of Curcuma aeruginosa Roxb. rhizome, Morinda citrifolia fruit and Apium graveolens leaf extract was investigated. All of extract was prepared by maceration with ethanol 70% and treated for antiinflammatory effect by using inducible-nitric oxide secretion measurement on lipopolysaccharide (LPS) - induce macrophage RAW 264.7 cells. We used three concentration of Curcuma aeruginosa Roxb. rhizome and Apium graveolens leaf extract at 25 ppm; 50 ppm; 100 ppm while for Morinda citrifolia fruit extract with 50 ppm; 100 ppm; 200 ppm. This study revealed that rhizome of Curcuma aeruginosa Roxb. and fruit of Morinda citrifolia extract inhibited inducible-nitric oxide synthesis with highest value of 84.2% at 25 ppm and 85,71% at 100 ppm in cells. However, leaf of Apium graveolens extract showed low inhibition with highest values of 18,85% at 100 ppm. This study demonstrates the potential of Curcuma aeruginosa Roxb. rhizome and Morinda citrifolia fruit as antiinflammatory agents.
Keywords: Curcuma aeruginosa Roxb., Apium graveolens, Morinda citrifolia, antiinflammation, inducible-nitric oxide, lipopolysaccharide-induce macrophage RAW 264.7 cells
Full Text:
PDFReferences
Cragg, G.M. and Newman, D.J., 2008, Detection, Isolation and Structural Determination in Bioactive Natural Products. 2nd Edition. CRCpress. Boca Raton, F.L.p. 324.
Hendra, R., Ahmad, S., Oskoueian., E., Sukari, A. and Shukor, M.Y., 2011, Antioxidant, Anti-inflammatory and Cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff Fruit, BMC Complement. Altern. Med., 11,10. CrossRef
Hsieh, Y.H., Kuo, P.M., Chien, S.C., Shyur, L.F. and Wang, S.Y., 2007, Eff ects of Chamaecyparis formosensis Matasumura Extractives on lipopolysaccharide-induced Release of Nitric Oxide, Phytomedicine, 14(10), 675–680. CrossRef
Moncada, S., Palmer, R.M. and Higgs, E.A., 1991, Nitric Oxide: Physiology, Pathophysiology, and Pharmacology, Pharmacol. Rev., 43(2), 109–142. Link
MacMicking, J., Xie, Q.W. and Nathan, C., 1997, Nitric Oxide and Macrophage Function, Ann Rev. Immunol., 15, 323–350. CrossRef
Oh, J.H., Lee, T.J., Park, J.W. and Kwon, T.K., 2008, Withaferin A inhibits iNOS Ixpression and Nitric Oxide Production by Akt Inactivation and Down-Regulating LPS-induced Activity of NF-kappaB in RAW 264.7 Cells, Eur. J. Pharmacol., 599(1-3), 11–17. CrossRef
Yang, E.J., Yim, E.Y., Song, G., Kim, G.O. and Gu Hyun, C., 2009, Inhibition of Nitric Oxide Production in Lipopolysaccharide-Activated RAW 264.7 Macrophages by Jeju Plant Extracts, Interdiscip. Toxicol.,2(4), 245-249. CrossRef
Yoon, W.J., Kim, S.S., Oh, T.H., Lee, N.H. and Hyun, C.G., 2009, Abies Koreana Essential Oinhibits Drug-Resistant Skin Pathogen Growth and LPS-Induced Inflammation of Murine Macrophage, Lipids, 44(5), 471–476. CrossRef
Wang, H. and Cao, Z.R., 2014, Anti-inflammatory Effects of (-)-Epicatechin in Lipopolysaccharide-Stimulated Raw 264.7 Macrophages, Trop. J. Pharm. Res., 3(9), 1415-1419. CrossRef
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev6iss3pp84-88
Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention
Indexed by:
Indonesian Society for Cancer Chemoprevention
